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复杂天然产物的生物合成和组合生物合成研究:Genetic characterization of the chlorothricin gene cluster...

  • 关键字: chlorothricin gene cluster 发布者:Jia XY, Tang J, Tang GL, Liu W 发布时间:2009-03-02 18:03:29 点击数: 5292次

Chem & Biol. 2006 13: 575-85
 
Genetic characterization of the chlorothricin gene cluster as a model for spirotetronate antibiotic biosynthesis.
 
 
State Key Laboratory of Bioorganic and Natural Product Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China.
 
Abstract. The biosynthetic gene cluster for chlorothricin (CHL) was localized to a 122 kb contiguous DNA from Streptomyces antibioticus DSM 40725, and its involvement in CHL biosynthesis was confirmed by gene inactivation and complementation. Bioinformatic analysis of the sequenced 111.989 kb DNA region revealed 42 open reading frames, 35 of which were defined to constitute the CHL gene cluster. An assembly model for CHL biosynthesis from D-olivose, 2-methoxy-5-chloro-6-methylsalicyclic acid, and chlorothricolide building blocks was proposed. This work represents cloning of a gene cluster for spirotetronate antibiotic biosynthesis and sets the stage to investigate the unusual macrolide biosynthesis including tandem Diels-Alder cyclizations, Baeyer-Villiger oxidation, and incorporation of an enoylpyruvate unit.