BNPC生命有机化学国家重点实验室
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姓名:康经武(Jingwu Kang)
职务:研究员、课题组长
E-mail:jingwu.kang@sioc.ac.cn
电话:86-21-54925385
传真:
点击数:5787

个人简介
1986.09-1990.06年毕业于陕西师范大学化学系,学士学位
1990.09-1996.12中科院兰州化学物理研究所,分析化学硕士和博士学位
1996.12-1999.05中科院兰州化学物理研究所,博士后,助理研究员
1999.06-2000.10比利时鲁汶大学药学院博士后
2000.10-2003.09在德国图宾根大学有机化学研究所事博士后
2003.09-至今 中科院上海有机化学研究所,“百人计划”研究员,课题组长

研究方向:生物分析与药物分析化学
我们课题组通过发展分析化学新技术和新方法帮助解决生命科学和药物研发中的重要科学问题。运用分析技术,如液相色谱、亲和色谱、毛细管电泳、质谱以及手性分离等解决药物筛选,药物靶标谱的鉴定以及蛋白糖基化修饰位点和糖链结构与功能分析方面的关键问题。这些问题的解决需要复杂体系的分离分析,涉及从有机小分子的结构和含量分析到生物大分子的结构与功能分析等分析化学技术和方法。

例如肝素(heparin) 和低分子量肝素是临床上普遍使用抗凝剂,主要用于血栓栓塞性疾病、心肌梗死、心血管手术、血液透析等,其全球年销售额已近百亿美元。但是由于其结构的高度复杂性,如多分散性、微观不均一性、强酸性等,肝素的结构与功能的关系至今仍未被完全阐释。我们在抗凝血药物依诺肝素钠结构分析研究方面取得了重要进展,发展了毛细管电泳结合超高效液相色谱-飞行时间质谱分析低分子量肝素钠精细结构的新方法获得了国际专利授权,引起了国际学术界和制药业的高度关注。这一成果也为杭州九源基因有限公司带来了巨大的经济效益。此外,我们发展的基于色谱分离和质谱鉴定的中药中有效成分的快速筛选方法,希望能帮助提升中药的质量控制标准和药理学研究。我们还致力于小分子药物靶标谱(target profiling)的鉴定方法研究。新药的成功离不开新靶点的发现和作用机制的阐明。这也是药理学和化学生物学研究的关键内容。只有全面表征药物与细胞内各蛋白成分的相互作用,才能预测可能的毒副作用,优化结构提高治疗效果,减小新药研发的巨大风险。然而,小分子药物靶标的鉴定却是一件令人生畏的工作,被形象地比作在一大堆干草中寻找一枚针。这些工作的开展既发展了分析化学学科,又解决了企业的实际需求。

代表性论文
[1] F. Li, Y. Zhang, D. Qiu, J. Kang, Screening of epidermal growth factor receptor inhibitors in natural products by capillary electrophoresis combined with high performance liquid chromatography-tandem mass spectrometry, J Chromatogr A, 1400 (2015) 117-123.

[2] Y. Zhang, F. Li, M. Li, J. Kang, Screening of mammalian target of rapamycin inhibitors in natural product extracts by capillary electrophoresis in combination with high performance liquid chromatography–tandem mass spectrometry, J Chromatogr A, 1388 (2015) 267-273.

[3] T. Wang, Q. Zhang, Y. Zhang, J. Kang, Screening of protein kinase inhibitors in natural extracts by capillary electrophoresis combined with liquid chromatography–tandem mass spectrometry, J Chromatogr A, 1337 (2014) 188-193.

[4] X. Zhang, T. Wang, H. Zhang, B. Han, L. Wang, J. Kang, Profiling of drug binding proteins by monolithic affinity chromatography in combination with liquid chromatography–tandem mass spectrometry, J Chromatogr A, 1359 (2014) 84-90.

[5] Q. Zhang, X. Chen, Z. Zhu, X. Zhan, Y. Wu, L. Song, J. Kang, Structural Analysis of Low Molecular Weight Heparin by Ultraperformance Size Exclusion Chromatography/Time of Flight Mass Spectrometry and Capillary Zone Electrophoresis, Anal Chem, 85 (2013) 1819-1827.

[6] C. Liu, J. Kang, Improved capillary electrophoresis frontal analysis by dynamically coating the capillary with polyelectrolyte multilayers, J Chromatogr A, 1238 (2012) 146-151.

[7] Z. Wang, C. Liu, J. Kang, A highly sensitive method for enantioseparation of fenoprofen and amino acid derivatives by capillary electrophoresis with on-line sample preconcentration, J Chromatogr A, 1218 (2011) 1775-1779.

[8] Z.M. Tang, Z.Y. Wang, J.W. Kang, Screening of acetylcholinesterase inhibitors in natural extracts by CE with electrophoretically mediated microanalysis technique, Electrophoresis, 28 (2007) 360-365.

[9] Z.-m. Tang, J.-w. Kang, Enzyme Inhibitor Screening by Capillary Electrophoresis with an on-Column Immobilized Enzyme Microreactor Created by an Ionic Binding Technique, Anal Chem, 78 (2006) 2514-2520.

[10] Z. Wang, Z. Tang, Z. Gu, Z. Hu, S. Ma, J. Kang, Enantioseparation of chiral allenic acids by micellar electrokinetic chromatography with cyclodextrins as chiral selector, Electrophoresis, 26 (2005) 1001-1006.

[11] J. Kang, D. Bischoff, Z. Jiang, B. Bister, R.D. Suessmuth, V. Schurig, A Mechanistic study of enantiomeric separation with vancomycin and balhimycin as chiral selectors by capillary electrophoresis. dimerization and enantioselectivity, Anal Chem, 76 (2004) 2387-2392.

[12] J. Kang, D. Wistuba, V. Schurig, A silica monolithic column prepared by the sol-gel process for enantiomeric separation by capillary electrochromatography, Electrophoresis, 23 (2002) 1116-1120.

[13] J.W. Kang, A. Van Schepdael, J.A. Orwa, E. Roets, J. Hoogmartens, Analysis of polymyxin B sulfate by capillary zone electrophoresis with cyclodextrin as additive Method development and validation, J Chromatogr A, 879 (2000) 211-218.



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