BNPC生命有机化学国家重点实验室
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姓名:唐功利 (GL Tang)
职务:博导 研究员
E-mail:gltang@mail.sioc.ac.cn
电话:021-54925113
传真:021-64166128
点击数:39455

课题组网站:http://tanggl.sioc.ac.cn

研究方向                                                                   
 天然产物的生物合成研究:以微生物来源的结构新奇、活性显著的复杂天然产物为目标分子,克隆其生物合成基因簇,从基因和蛋白水平揭示自然界神奇的化学过程,理解其中蕴涵的新型酶催化反应,在此基础上调控其生物合成途径来创造“非天然”天然产物分子多样性。
 抗生素代谢工程与菌种改良:以抗生素的生物合成途径为基础,采用代谢工程的方法和策略致力于提高目标化合物的产量,改变发酵产物的组份及产生新化合物。
 活性天然产物靶蛋白的筛选:以噬菌体展示克隆为基础优化方法,并利用该方法筛选一些高活性天然产物的生物靶分子,进而理解其生物活性的机理。

工作经历                                                                   
 2003年8月至今:中国科学院上海有机化学研究所,研究员
 2001年8月至2003年8月:美国威斯康星大学麦迪逊分校药学院,研究助理
 2000年9月至2001年8月:美国美国加州大学戴维斯分校化学系,博士后,博士后
 1999年8月-2000年8月:中国科学院上海有机化学研究所助理研究员、副研究员

教育背景                                                                   
 1994年9月至1999年7月:中国科学院上海有机化学研究所,理学博士,导师:陈海宝研究员。
 1990年9月至1994年7月:南开大学环境科学系,理学学士,导师:黄国兰教授。

主要获奖情况                                                               
 中国科学院“伟华科技奖”(1999年)。

代表性发表论文                                                             
1. Pu, J.-Y.; Chao, P.; Tang, M.-C.; Zhang, Y.; Guo, J.-P.; Song, L.-Q.; Hua, Q.; Tang, G.-L.* (2013) Naphthyridinomycin biosynthesis revealing the use of leader peptide to guide nonribosomal peptide assembly. Org. Lett. in press (doi: 10.1021/ol401549y).

2. Pan, H.-X.; Chen, Z.-Z.; Shao, L.; Li, J.-A.; Chen, J.-S.; Zhu, C.-B.; Tang, G.-L.*, Chen, D.-J.* (2013) Production of ramoplanin analogues by genetic engineering of Actinoplanes sp. Biotechnol. Lett. in press (doi: 10.1007/s10529-013-1261-z).

3. Ma, H.-M.; Zhou, Q.; Tang, Y.-M.; Zhang, Z.; Chen, Y.-S.; He, H.-Y.; Pan, H.-X.; Tang, M.-C.; Gao, J.-F.; Zhao, S.-Y.; Igarashi, Y.; Tang, G.-L.* (2013) Unconventional origin and hybrid system for construction of pyrrolopyrrole moiety in kosinostatin biosynthesis. Chem. Biol. 20, 796-805.

4. Li, J.-E.; Guo, Z.-Y.; Huang, W.; Meng, X.-X.; Ai, G.-M.; Tang, G.-L.*, Chen, Y.-H.* (2013) Mining of a of a streptothricin gene cluster from the Streptomyces sp. TP-A0356 genome via heterologous expression. Sci. China Life Sci. 56, 619-627.

5. Tang, M.-C.; He, H.-Y.; Zhang, F.; Tang, G.-L.* (2013) Baeyer-Villiger oxidation of acyl carrier protein-tethered thioester to acyl carrier protein-linked thiocarbonate catalyzed by a monooxygenase domain in FR901464 biosynthesis. ACS Catal. 3, 444-447.

6. Pan, H.-X.; Li, J.-A.; Shao, L.; Zhu, C.-B.; Chen, J.-S.; Tang, G.-L.*, Chen, D.-J.* (2013) Genetic manipulation revealing an unusual N-terminal region in a stand-alone non-ribosomal peptide synthetase involved in the biosynthesis of ramoplanins. Biotechnol. Lett. 35, 107-114.

7. He, H.-Y.; Pan, H.-X.; Wu, L.-F.; Zhang, B.-B.; Chai, H.-B.; Liu, W.; Tang, G.-L.* (2012) Quartromicin biosynthesis: two alternative polyketide chains produced by one polyketide synthase assembly line. Chem. Biol. 19, 1313-1323.

8. Xu, H.; Huang, W.; He, Q.-L.; Zhao, Z.-X.; Zhang, F.; Wang, R.; Kang, J.; Tang, G.-L.* (2012) Self-resistance to antitumor antibiotic: a DNA glycosylase triggers the base excision repair system in yatakemycin biosynthesis. Angew. Chem. Int. Ed. 51, 10532-10536. (Highlighted in Nat. Chem. Biol. 2012, 8, 873).

9. Chen, Y.-L.; Zhao, J.; Liu, W.; Gao, J.-F.; Tao, L.-M.; Pan, H.-X.*; Tang, G.-L.* (2012) Identification of phoslactomycin biosynthetic gene clusters from Streptomyces platensis SAM-0654 and characterization of PnR1 and PnR2 as positive transcriptional regulators. Gene, 509, 195-200.

10. Huang, W.; Xu, H.; Li, Y.; Zhang, F.; Chen, X.-Y.; He, Q.-L.; Igarashi, Y.; Tang, G.-L.* (2012) Characterization of yatakemycin gene cluster revealing a radical S-adenosylmethionine dependent methyltransferase and highlighting spirocyclopropane biosynthesis. J. Am. Chem. Soc. 134, 8831-8840.

11. Peng, C.; Pu, J.-Y.; Song, L.-Q.; Jian, X.-H.; Tang, M.-C.; Tang, G.-L.* (2012) Hijacking a hydroxyethyl unit from a central metabolic ketose into a nonribosomal peptide assembly line. Proc. Natl. Acad. Sci. U.S.A. 109, 8540-8545.

12. Jian, X.-H.; Pan, H.-X.; Ning, T.-T.; Shi, Y.-Y.; Chen, Y.-S.; Li, Y.; Zeng, X.-W.; Xu, J.; Tang, G.-L.* (2012) Analysis of YM-216391 biosynthetic gene cluster and improvement of the cyclopeptide production in a heterologous host. ACS Chem. Biol. 7, 646-651.

13. Tang, M.-C.; Fu, C.-Y.; Tang, G.-L.* (2012) Characterization of SfmD as a heme peroxidase that catalyzes the regioselective hydroxylation of 3-methyltyrosine to 3-hydroxy-5- methyltyrosine in saframycin A biosynthesis. J. Biol. Chem. 287, 5112-5121.

14. Peng, C.; Tang, Y.-M.; Li, L.; Ding, W.; Deng, W.; Pu, J.-Y.; Liu, W.; Tang, G.-L.* (2012) In vivo investigation of the role of SfmO2 in saframycin A biosynthesis by structural characterization of the analogue saframycin O. Sci. China Chem. 55, 90-97.

15. Zhang, F.; He, H.-Y.; Tang, M.-C.; Zhou, Q.; Tang, G.-L.* (2011) Cloning and elucidation of the FR901464 gene cluster revealing a complex acyltransferase-less polyketide synthase using glycerate as starter units. J. Am. Chem. Soc. 133, 2452-2462.

16. Wang, J.-B.; Pan, H.-X.; Tang, G.-L.* (2011) Production of doramectin by rational engineering of the avermectin biosynthetic pathway. Bioorg. Med. Chem. Lett. 21, 3320-3323.

17. Fu, C.-Y.; Tang, M.-C.; Peng, C.; Li, L.; He, Y.-L.; Liu, W. Tang, G.-L.* “Biosynthesis of 3-hydroxy-5-methyl-O-methyltyrosine in the saframycin/ safracin biosynthetic pathway”, J. Microbiol. Biotechnol. 2009, 19, 439-446.

18. He, Q.-L.; Jia, X.-Y.; Tang, M.-C.; Tian, Z.-H.; Tang, G.-L.*; Liu, W.* “Dissection of two acyl transfer reactions centered on acyl-S-carrier protein intermediates for incorporating 5-chloro-6-methyl-O-methylsalicyclic acid into chlorothricin”, ChemBioChem 2009, 10, 813-819.

19. He, Q.-L.; Jiang, H.; Zhang, F.; Chen, H.-B.; Tang, G.-L.* “Simultaneous identification of multiple receptors of natural product using an optimized cDNA phage display cloning”, Bioorg. Med. Chem. Lett. 2008, 18, 3995-3998.

20. Li, L.; Deng, W.; Song, J.; Ding, W.; Zhao, Q.-F.; Peng, C.; Song, W.-W.; Tang, G.-L.*; Liu, W.* “Characterization of the saframycin A gene cluster from Streptomyces lavendulae NRRL 11002 revealing a NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner”, J. Bacteriol. 2008, 190, 251-263.

21. Zhao, Q.; He, Q.; Ding, W.; Tang, M.; Kang, Q.; Yu, Y.; Deng, W.; Zhang, Q.; Fang, J.; Tang, G.-L.; Liu, W.* “Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis”. Chem. Biol. 2008, 15, 693-705.

22. Fang, J, Zhang, Y, Huang, L, Jia, X, Zhang, Q, Zhang, X, Tang, G.-L., Liu W*. “Cloning and characterization of the tetrocarcin A gene cluster from M. chalcea NRRL 11289 reveals a highly conserved strategy for tetronate biosynthesis in spirotetronate antibiotics”. J. Bacteriol. 2008, 190, 6014-25.

23. Tang, G.-L.; Cheng, Y.-Q.; Shen, B.  “Chain initiation in the leinamycin-producing hybrid nonribosomal peptide/polyketide synthetase from S. atroolivaceus S-140”, J. Biol. Chem. 2007, 282, 20273-20282.

24. Jia, X.-Y.; Tian, Z.-H.; Shao, L.; Qu, X.-D.; Zhao, Q.-F.; Tang, J.; Tang, G.-L.*; Liu, W.* “Characterization of the chlorothricin biosynthetic gene cluster as a model for spirotetronate antibiotic biosynthesis”, Chem. Biol. 2006, 13, 575-585.

25. Shao, L.; Qu, X.-D.; Jia, X.-Y.; Zhao, Q.-F.; Tian, Z.-H.; Wang, M.; Tang, G.-L.*; Liu, W* “Cloning and characterization of a bacterial iterative type I polyketide synthase gene encoding the 6-methylsalicyclic acid synthase”, Biochem. Biophy. Res. Comm. 2006, 345, 133-139.

26. Tang, G.-L.; Cheng, Y.-Q.; Shen, B. “Polyketide chain skipping mechanism in the biosynthesis of the hybrid nonribosomal peptide-polyketide antitumor antibiotic leinamycin in S. atroolivaceus S-140”. J. Nat. Prod. 2006, 69, 387-393.

27. Tang, G.-L.#; Cheng, Y.-Q.#; Shen, B. “The biosynthetic gene cluster of the antitumor antibiotic leinamycin from S. atroolivaceus S-140 revealing unprecedented architectural complexity for a hybrid non-ribosomal peptide/ polyketide synthetase”, Chem. Biol., 2004, 11, 33-45.

28. Cheng, Y.-Q.#; Tang, G.-L.#; Shen, B. “Type I polyketide synthase requiring a discrete acyltransferase for polyketide biosynthesis. (#contribution equally)”, Proc. Natl. Acad. Sci. USA. 2003, 100, 3149-3154. [Featured in: Proc. Natl. Acad. Sci. USA. 2003, 100, 3010-3012]

29. Cheng, Y.-Q.; Tang, G.-L.; Shen, B. “Identification and localization of the antitumor macrolactam leinamycin gene cluster from S. atroolivaceus S-140”, J. Bacteriol. 2002, 184, 7013-24.

30. Tang, G.-L.; Wang, Y.-F.; Bao, J.-S.; Chen, H.-B. “Two-cistron system overexpression of chloroplast glyceraldehyde-3-phosphate dehydrogenase subunit B and B-derivatives from spinach in E. coli.”, Protein Expression Purif. 2001, 22, 31-37.

31. Tang, G.-L.; Wang, Y.-F.; Bao, J.-S.; Chen, H.-B. “Overexpression in E. coli and characterization of the chloroplast fructose-1,6-bisphosphatase from wheat”, Protein Expression Purif. 2000, 19, 411-418.

 



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