BNPC生命有机化学国家重点实验室

个人简介：

1989-1993 南京大学少年班，分析化学专业，学士；

1993-1995 南京化工厂，分析技术员；

1995-2000 中科院上海有机所，生物有机专业，博士；

2000-2001 中科院上海有机所，助理研究员；

2001-2006 美国密歇根大学，结构生物学，博士后；

2006-present 中科院上海有机所，蛋白质晶体学与化学生物学，研究员。

研究方向：

周佳海实验室着重选择与重要疾病相关的蛋白质及具有新颖催化性质的酶进行结构生物学和化学生物学研究，利用各种生物化学、生物物理方法（特别是高分辨X-射线晶体学技术）作为研究手段，希望通过结构测定和功能研究阐明蛋白-配基相互作用及酶促反应机制，从而为新药设计和酶的改造奠定基础。

我们以生命有机化学国家重点实验室为依托，与中国协和医科大学、中科院上海生化细胞所、复旦大学、上海交通大学、华南理工大学等多家单位建立了广泛的合作关系，课题研究涉及与系统性红斑狼疮、乙型肝炎等重要疾病相关蛋白质的结构与功能关系和天然产物生物合成途径的酶学机制等方面。

主要工作成就：

国家自然科学基金委“面上青年科学基金”项目，2008-2010；

国家自然科学基金委“面上基金”项目，2009-2011；

中科院研究员项目，2008-2011；

“国家重大科学研究计划”项目蛋白质研究973课题，2009-2013；

“国家科技重大专项” 项目病毒性肝炎课题，2008-2010。

近年来发表论文:

1. Li J.^*, Wang C.^*, Zhang Z.M., Cheng Y.Q.^#, Zhou J.^# (2014) The structural basis of an NADP⁺-independent dithiol oxidase in FK228 biosynthesis. Scientific Reports, in press.DOI: 10.1038/srep04145

2. Zhang Z.M.^*, Yang F.^*, Zhang J., Tang Q., Li J., Gu J., Zhou J.^#, Xu Y.Z.^# (2013) Crystal structure of Prp5p reveals interdomain interactions that impact spliceosome assembly. Cell Reports, 5:1269-1278.

3. Xu Z., Li, S., Li J., Li Y., Feng X., Wang R., Xu H.^#, Zhou J.^# (2013) The structural basis of Erwinia rhapontici isomaltulose synthase. PLoS ONE, 8:e74788.

4. Ma J.^*, Wu L.^*, Guo F., Gu J., Tang X., Jiang L., Liu J., Zhou J.^#, Yu H^#. (2013) Enhanced enantioselectivity of a carboxyl esterase from Rhodobacter sphaeroides by directed evolution. Applied Microbiology and Biotechnology, 97:4897-4906.

5. Yang F., Wang X.Y., Zhang Z.M., Pu J., Fan Y.J., Zhou J., Query C.C., Xu Y.Z. (2013) Splicing proofreading at 5’splice sites by ATPase Prp28p. Nucleic Acids Research, 41:4660-4670.

6. Sun Y.^*, Song H.^*, Li J., Li Y., Jiang M., Zhou J.^#, Guo Z.^# (2013) Structural basis of the induced-fit mechanism of 1,4-dihydroxy-2-naphthoyl coenzyme A synthase from the crotonase fold superfamily. PLoS ONE, 8:e63095.

7. Zhang Z.M.^*, Zhang X.^*, Zhou Z.R., Hu H.Y., Liu M., Zhou B.^#, Zhou J.^#(2013) NMR Structure Note: Solution structure of the Magnaporthe oryzae avirulence protein AvrPiz-t. Journal of Biomolecular NMR, 55:219-223.

8. Sun Y., Song H., Li J., Jiang M., Li Y., Zhou J.^#, Guo Z.^# (2012) Active site binding and catalytic role of bicarbonate in 1,4-dihydroxy-2-naphthoyl coenzyme A synthases from vitamin K biosynthetic pathways. Biochemistry, 51:4580-4589.

9. Diao L., Dong Q., Xu Z., Yang S., Zhou J.^#, and Freudl R.^# (2012) Functional implementation of the posttranslational SecB-SecA protein targeting pathway in Bacillus subtilis. Applied and Environmental Microbiology, 78:651-659.

10. Ren A., Xia Z.X., Yu W., Zhou J. # (2010) Expression, crystallization and preliminary X-ray analysis of an anomeric inverting agarase from Pseudoalteromonas sp. CY24. Acta Crystallographica Section F: Structural Biology Communications 66:1635-1639.

11. Zheng C., Liu H.H., Yuan S., Zhou J. and Zhang B. (2010) Molecular basis of LMAN1 in coordinating LMAN1-MCFD2 cargo receptor formation and ER-to-Golgi transport of FV/FVIII. Blood, 116:5698-5706.

12. Zheng C., Liu H.H., Zhou J, and Zhang B. (2010) EF-hand domains of MCFD2 mediate interactions with both LMAN1 and coagulation factor V or VIII. Blood 115:1081-1087.

13. Xiao J., Xia H., Zhou J., Azmi I., Davies B.A., Katzmann D.J. and Xu Z. (2008) Structural basis of Vta1 function in the multi-vesicular body sorting pathway. Developmental Cell, 14: 37-49.

14. Zhou J.^*, Liu C.Y.^*, Back S.H., Clark R.L., Peisach D., Xu Z.^# and Kaufman R.J.^# (2006) The crystal structure of human IRE1 luminal domain reveals a conserved dimerization interface required for activation of unfolded protein response. Proceedings of the National Academy of Sciences USA,103:14343-14348.

15. Zhou J. and Xu Z. (2005) The structural view of bacterial translocation-specific chaperone SecB: implications for function, Molecular Microbiology, 58:349-357.

16. Zhou J. and Xu Z. (2003) Structural determinants of SecB recognition by SecA in bacterial protein translocation, Nature Structural Biology,10:942-947.

*same contribution, #co-correspondence

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